TITLE:
Expression of Genes Affecting Skin Coloration and Sugar Accumulation in Apple Fruits at Ripening Stages in High Temperatures
AUTHORS:
Seon Ae Kim, Soon Young Ahn, Hyun Hee Han, In Chang Son, Hae Keun Yun
KEYWORDS:
Anthocyanin, Apple, High Temperature, Skin Coloration, Sugar Content
JOURNAL NAME:
World Journal of Engineering and Technology,
Vol.3 No.3B,
August
25,
2015
ABSTRACT: High temperature is one of the most
important environmental factors that affect the successful cultivation of apple
(Malus × domestica Borkh). The expression of genes related with anthocyanin
synthesis and sugar accumulation by high temperature treatment was investigated
in the fruits of “Fuji” apple at different developmental stages in different
temperature conditions through real- time PCR. In the initial ripening stages
in fruits, there was high expression of genes associated with fruit ripening,
flavonoid compound accumulation, and coloration in high temperatures. Expression
of phenylalanine ammonia-lyase, chalcone synthase, flavone 3-hydrogenase, and
malate dehydrogenase genes increased gradually in initial ripening stages,
while sharply reduced at 24 hr after treatment. In addition, there was no significant
difference in the expression of all temperature treatments in late ripening
stages. In the further work, investigation of expression levels of various
genes could be conducted in the level of transcriptomes from fruits at the
early stages to get meaningful information of ripening metabolism in apples in high
temperatures.