TITLE:
Nephroprotective Effect of Nigella Sativa and Matricaria Chamomilla in Cisplatin Induced Renal Injury—Supportive Treatments in Cisplatin Nephrotoxicity
AUTHORS:
Ragaa H. M Salama, Nahed A Abd-El-Hameed, Sary KH Abd-El-Ghaffar, Zaghloul T. Mohammed, Nagwa M. A Ghandour
KEYWORDS:
Cisplatin, Nephrotoxicity, Nephroprotection, Matricaria Chamomilla, Nigella Sativa, Vitamin E
JOURNAL NAME:
International Journal of Clinical Medicine,
Vol.2 No.3,
July
13,
2011
ABSTRACT: Nigella sativa and Matricaria chamomilla are extensively consumed as tea or tonic. Despite their widespread use as a home remedy, relatively few trials evaluated their benefits in nephroprotection. Hence, this study evaluates the nephroprotective effects of supportive treatments (N. sativa, M. chamomilla and vitamin E) in cisplatin nephrotoxicity rat model. Eighty rats divided into 10 groups, of 8 animals each. The first group (G1) injected with saline intra-pretoneal (i.p). G2 injected with 5 mg/kg cisplatin i.p on zero day of experiment and repeated 4 times, with 5 days free interval. G3-G10 received daily supportive treatments, started 5 days before the experiment (–5day). Concomitantly G4, G6, G8 and G10 injected with 5 mg/kg cisplatin i.p like G2. On day sixteen, animal scarified, serum and/or kidney tissue were used to determine kidney function tests (serum urea, creatinine, NAG, β-gal), oxidative stress indices (NO, LPO), antioxidant activities (SOD), sulphur compounds (GGT, GSH, total thiols ), apoptotic indices (cathepsin D, DNA fragmentation), two minerals (Ca2+ and zn2+). Cisplatin caused marked elevation in serum GGT that reduced signifi-cantly in group received M. chamomilla with cisplatin (P