TITLE:
In Vitro Propagation of Grapevine Cultivars with Potential for South of Brazil
AUTHORS:
Jean Carlos Bettoni, Murilo Dalla Costa, João Peterson Pereira Gardin, Aike Anneliese Kretzchmar, Juliana Aparecida Souza
KEYWORDS:
IAC 571-6, Poloskei Muskotaly, In Vitro Culture, Vitis, Culture Medium
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.6 No.11,
July
28,
2015
ABSTRACT: The micropropation is an important biotechnological tool for obtaining and maintaining mother vine plants with high quality plant health. The objective was to evaluate the establishment and multiplication in vitro and ex vitro acclimatization of grape genotypes with potential for Southern Brazil. Vine nodal segments were cultured in five culture medium formulations without adding growth regulators. It was evaluated the number of leaves and roots, length of roots and shoots, replication rate, relative chlorophyll index, percentage of regenerated and rooted plants, dry biomass of shoot, root and total plants grown in vitro and after acclimatization. In vitro propagation of IAC 571-6 rootstock and cv. Poloskei Muskotaly through nodal segments provided high rates of regeneration and rooting. High survival rates were obtained in the acclimatization of IAC 571-6 and P?l?skei Muskotaly. Considering all the variables, the culture medium Roubelakis showed the best growth rates and development for shoots and roots, and in vitro multiplication rate for IAC 571-6 and Poloskei Muskotaly grape varieties.