TITLE:
Molecular Analysis of Clerodendrum formicarum Effects in Painful Diabetic Neuropathy in Rat
AUTHORS:
Ghislain Loubano-Voumbi, Mor Diaw, Valentin Ouedraogo, Abdou Khadir Sow, Aïssatou Seck, Luc Magloire Anicet Boumba, Abdoulaye Ba, Abdoulaye Samb
KEYWORDS:
Clerodendrum formicarum, Astrocytes, Microglial Cells, Diabetic Neuropathy Pain, Cyclooxygenase
JOURNAL NAME:
World Journal of Neuroscience,
Vol.5 No.4,
July
27,
2015
ABSTRACT: The pathophysiology of
diabetic neuropathic pain is due to primarily metabolic and vascular factors.
There is an increase in sorbitol and fructose, glycated end products, reactive
oxygen species and activation of protein kinase C in the diabetic state. All
these factors lead to direct damage to the nerves. Taking effective clinical
management of neuropathic pain is based on a pharmacological treatment that has
shown their limits and many side effects. The hypothesis of central
sensitization inhibited by Clerodendrum
formicarum, an African pharmacopoeia plant used to treat headaches,
arthritis, epilepsy and chronic pain could act on astrocytes and microglial
cells. The objective of this work is to study the effect of Clerodendrum formicarum (100, 150 and 200 mg/kg body weight)
on astrocytes and microglial cells in a model of diabetic neuropathic pain
induced by alloxan monohydrate (150 mg/kg). We noted a suppression of
mechanical allodynia and mechanical hyperalgesia respectively by the Von Frey
filaments test and the pressure test on the paw by the Clerodendrum formicarumextracts
(ECF) at different doses from 2 h at the first injection of the ECF. After 5
days of treatment, we expressed by Western Blot bands of different proteins and
by quantitative RT-PCR, we determined inhibition of the expression of GFAP,
CD11b and isoforms 1 and 2 of cyclooxygenase. These results suggest that ECF
inhibits the activation of astrocytes, microglial cells and cyclooxygenase
signaling pathway.