TITLE:
Genotyping of New and Old Proteus mirabilis Isolates from Mansoura Hospitals in Egypt by rpoB Sequence Analysis
AUTHORS:
Mohamed Mohamed Adel El-Sokkary
KEYWORDS:
Proteus, Genetic Analysis, rpoB Sequencing, Ribotyping
JOURNAL NAME:
Advances in Microbiology,
Vol.5 No.7,
July
27,
2015
ABSTRACT: Sequence analysis of the
RNA polymerase B subunit encoding gene (rpoB) has been proposed as a useful
tool for bacterial identification. This method has been implemented to differentiate
five well-defined Proteus species: P. mirabilis, P. hauseri, P. penneri, P. vulgaris, and P.
myxofaciens. In this study, we evaluated the usefulness of rpoB sequencing for intraspecies discrimination
of P. mirabilis. The
sequence of rpoB 909 bp region was analyzed in 15 newly isolated strains
and 5 of 8 years old isolates from different clinical sources. Three respective
groups were obtained. The first group included 13 of the new strains showing
similarity with Proteus mirabilis (ATCC 29906) strain. The second group including 3 of the old strains differs from
the first group with a divergence of 0.22%. Group 3 contains only a single new strain
33. The sequence of this strain shows differences in the rpoB 909 bp region analyzed from the members
of group 1 and the second group by 1.65% and 1.87% divergence respectively. According
to our results, genetic differences could be detected within the P. mirabilis species. Therefore much
more effort should be made to re-evaluate rpoB method and validate its usefulness
as a molecular diagnostic method.