TITLE:
Application of on-line quality control for salvianolic acid B by near infrared spectroscopy
AUTHORS:
Jin-Wei Zhang, Yan Liu, Wei-Wei Liu, Yan-Ying Zhang
KEYWORDS:
NIR; Salvianolic Acid B; On-Line Quality Control; Industrial Scale
JOURNAL NAME:
Health,
Vol.1 No.3,
December
1,
2009
ABSTRACT: OBJECTIVE: To study and establish quality con-trol model of the Salvianolic Acid B by Near In-frared Spectroscopy (NIRS), and to realize on-line quality control of extracting and purifying proc-esses of industrial scale herbal product manu-facturing. METHOD: NIR chromatography was obtained from on-line NIR detection of extract-ing process and purifying process. HPLC analysis was carried out to determine the con-tents of salvianolic acid B. Partial Least Squares Regression (PLS) was used to establish the model between the information between NIRS and HPLC. RESULTS: For extracting model: the optimum Near Infrared (NIR) wavelength range was 9815- 5430cm-1, R=0.9784, RMSEC=0.258; for puri-fying model: the optimum NIR wavelength range was 9815-5430cm-1, R=0.9776, RMSEC=4.02. The average relative error was