TITLE:
Dual PINK Mutant and Aβ42-Dependent Lifespan Shorten and Flight Impairment in Transgenic Drosophila Partially Alleviates by a Lactococcus lactis Supplemented Diet
AUTHORS:
Dong Gyun Ko, Young Bum Eun, Jong Uk Na, Sang-Tae Kim
KEYWORDS:
Drosophila melanogaster, Lactococcus lactis, PINK1, Lifespan, Ommatidia, Aβ42
JOURNAL NAME:
Journal of Behavioral and Brain Science,
Vol.5 No.7,
July
10,
2015
ABSTRACT: Oxidative stress
has been strongly related with Parkinson disease (PD) and Alzheimer disease
pathogenesis. We determined the effects of Lactococcus
lactis (LAL) supplementation on the generated loss-of-function mutants of PINK1
B9, an AR-JP-linked gene and Aβ42 induced
phenotypes in a Drosophila melanogaster model of PD/AD. Enhanced mutant
PINK1 B9 and Aβ42 expression in D.
melanogaster dopaminergic (DA) neurons can curtail lifespan, flight muscle
accompanied by locomotive defects and we have observed longevity methods to
assay the effects of LAL on D. melanogaster survival. Furthermore, flies
expressing mutant PINK1 B9 and Aβ42
in their brain fed LAL had up to the two weeks, or 25%, greater median lifespan
than those fed a standard sucrose diet. In addition, LAL improved mutant PINK1
B9 and Aβ42-induced flight
impairments in the Drosophila wing. Our microscopy analyses revealed that individuals
fed LAL had improved atypical ommatidia as well as an increased thirteen percentage
of flight ability than those fed a control diet. We propose that LAL, rich in
naturally occurring probiotics and antioxidants, promotes the survival of
neurons in brain and wing muscle tissues with increased levels of mutant PINK1
B9 and Aβ42 via a protective cell
survival mechanism.