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Levi, B., Wan, D., Glotzbach, J., Hyun, J., Januszyk, M., Montoro, D., Sorkin, M., James, A., Nelson, E., Li, S., Quarto, N., Lee, M., Gurtner, G. and Longaker, M. (2011) CD105 Protein Depletion Enhances Human Adipose-Derived Stromal Cell Osteogenesis through Reduction of Transforming Growth Factor B1 (TGF-B1) Signaling. The Journal of Biological Chemistry, 286, 39497-39509.
http://dx.doi.org/10.1074/jbc.M111.256529

has been cited by the following article:

• TITLE: Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) with Gelatin Solvent

  AUTHORS: Nike Hendrijantini, Utari Kresnoadi, Sherman Salim, Bambang Agustono, Endang Retnowati, Iwan Syahrial, Pungky Mulawardhana, Manggala Pasca Wardhana, Coen Pramono, Fedik Abdul Rantam

  KEYWORDS: Human Umbilical Cord Mesenchymal Stem Cells, Mesenchymal Stem Cells, Phenotypic Characterization, Osteogenic Differentiation, Bone Tissue Engineering

  JOURNAL NAME: Journal of Biomedical Science and Engineering, Vol.8 No.7, July 9, 2015

  ABSTRACT: The human umbilical cord is a source of numerous Mesenchymal Stem Cells (MSCs), making it as a potential source of allogeneic multipotent cell for bone tissue engineering. The aims of this study were to find: 1) Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) phenotypic characterization, 2) The in-vitro osteogenic differentiation potential of hUCMSCs, 3) The cytotoxicity of gelatin solvent to hUCMSCs using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. As a result, through characterization of hUCMSCs, the majority of target cells expressed specific MSCs markers, Cellular Differentiation (CD)73, smaller number of subpopulation expressed CD90 with only minimal subpopulation expressed CD105 and all negative MSCs markers. Osteoblastic differentiation was found in a significantly high number of cells when in vitro osteogenic differentiation of hUCMSCs with Alizarin Red staining was done. Biocompatibility analysis using the MTT assay showed that gelatin solvent and Alpha modification of minimum essential medium Eagle (α-MEM) was non-toxic for hUCMSCs in vitro. The study concluded that hUCMSCs isolated from human umbilical cord was capable of undergoing in vitro osteogenesis, indicating its potential as allogeneic stem cells for clinical application in bone tissue engineering.