TITLE:
Optimization of Culture Conditions for Production of Bioactive Metabolites by Streptomyces spp. Isolated from Soil
AUTHORS:
Sunita Bundale, Deovrat Begde, Nandita Nashikkar, Tukaram Kadam, Avinash Upadhyay
KEYWORDS:
Streptomyces, Bioactive Metabolites, Culture Conditions
JOURNAL NAME:
Advances in Microbiology,
Vol.5 No.6,
June
25,
2015
ABSTRACT: The current work was
carried out under a screening program targeted at isolation of bioactive Streptomyces species from soil samples.
A total of 54 Streptomyces species
were isolated from soil samples, out of which 4 isolates were found to be
promising. These isolates were identified as Streptomyces spectabilis, Streptomyces
purpurascens, Streptomyces
coeruleorubidus and Streptomyces
lavendofoliae and their sequences have been deposited in the GenBank. The
influence of culture conditions including, incubation time, incubation
temperature, initial pH and different carbon and nitrogen sources on growth and
bioactive compound formation was investigated. Isolate R1, identified as Streptomyces spectabilis, showed
maximum bioactive metabolite production with cellobiose and peptone as the
carbon and nitrogen sources, on the 5th day at pH 5 at 30℃. The optimum conditions for production by isolate R3, identified as Streptomyces
purpurascens, were observed to be starch and casein as the carbon and
nitrogen sources, pH 7, temperature 30℃ and an incubation period of eight
days. For isolate R5, identified as Streptomyces coeruleorubidus,
maximal production resulted on the sixth day at pH 6 and temperature of 35℃ with mannitol and JBM. Isolate Y8, identified as Streptomyces
lavendofoliae, was found to produce high levels of bioactive metabolites in
the medium supplemented with starch and peptone on the 10th day at
pH 7 and at an incubation temperature of 30℃. The four strains tested
here behaved differently, each one requiring specific conditions for maximum
growth as well as bioactive metabolite production.