TITLE:
Cold-Resistant Breeding of Oenothera speciosa Using Silico Ion Implantation
AUTHORS:
Yan Ma, Yan Lin, Liping Sun, Lina Song, Rongfeng Cui, Yibin Qie
KEYWORDS:
Silico Ion Implantation, Oenothera speciosa, Cold Resistant Breeding
JOURNAL NAME:
Open Journal of Forestry,
Vol.5 No.5,
June
24,
2015
ABSTRACT: Oenothera speciosa, belonging to thermophilous plant, cannot overwinter in Beijing. To enhance the
overwintering rate of Oenothera speciosa, the seeds were treated through silico ion implantation (SII),
with five various fluence ranges (1 × 109 - 1 × 1011 ions/cm2) of 40 MeV and
four various fluence ranges (1 × 1010 - 5 × 1011 ions/cm2) of 35 MeV, respectively. M1 generations
of various SII-treated Oenothera speciosa lines can overwinter, and the highest overwinter rate (41.3%) was observed in Oenothera speciosa lines treated with 35 MeV and fluence 5 × 1010 ions/cm2. M2 and M3 generations of all treated lines were able to overwinter
smoothly. The results indicated that SII treatment can enhance the
cold-resistance of Oenothera speciosa heritably. Furthermore, physiological indexes including relative electrical
conductivity, MDA contents and proline contents of SII-treated Oenothera
speciosa pot seedlings were
detected after low temperature stress. The results revealed that relative
electrical conductivities and MDA contents of M1, M2 and
M3 generations of SII-treated Oenothera speciosa plants were lower than that of control,
whereas the proline contents were higher than control in the LJ°C cold
stress. Taken together, the cold resistance of SII-treated Oenothera
speciosa plants was improved,
which made it possible to be used as a perennial flower in landscaping in
Beijing.