TITLE:
Sanitizing Method Effects on Depending-Culture Microorganisms in Tuber aestivium
AUTHORS:
Ilef Ben Romdhane, Neila Saidi, Shweta Deshaware, Salem Shamekh
KEYWORDS:
Tuber aestivium Ascocarps, Total Count Mesophilic Bacteria, Molds and Yeast Decontamination
JOURNAL NAME:
Advances in Microbiology,
Vol.5 No.6,
June
19,
2015
ABSTRACT: Tuber aestivum/uncinatum has been widely used as food, food additives, and traditional medicine.
Truffles are extremely perishable with a short postharvest life quality
requiring a special handling for marketing in order to delay its deterioration.
This study aimed to assess the effects of different sanitizing methods on superficial Tuber aestivium quality ascocarp. The results showed that the
best treatment was obtained by immersing the Truffle ascocarps in boiling water
for 1 or 2 min where counts of total mesophilic microoraganisms (TMM) were
respectively 81 and 7 CFU per g of dry Truffle ascocarps biomass, respectively.
However, the highest TMM was obtained after rinsing Truffle ascocarps in 2%
NaOH where recovery was 108 CFU per g of dry Truffle ascocarps
biomass. Treatments applied to disinfect Truffle ascocarps were classified by
increasing degree of efficiency as follows to reduce the microbial load
expressed in CFU/g: Dipping in boiling water (2 min) 7 ± 3.41; dipping in
boiling water (1 min) 81 ± 25.8, rinsing with alcohol 2.102 ± 13;
rinsing with tap water 6.103 ± 36; rinsing with H2O2 6.104 ± 2; brushing 2.105 ± 28 and rinsing with NaOH 108 ± 15.