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Claesson, M.J., Wang, Q., O’Sullivan, O., Greene-Diniz, R., Cole, J.R., Ross, R.P. and O’Toole, P.W. (2010) Comparison of Two Next-Generation Sequencing Technologies for Resolving Highly Complex Microbiota Composition Using Tandem Variable 16S rRNA Gene Regions. Nucleic Acids Research, 38, e200. http://dx.doi.org/10.1093/nar/gkq873

has been cited by the following article:

• TITLE: Identification of Bacterial Fish Pathogens in Brazil by Direct Colony PCR and 16S rRNA Gene Sequencing

  AUTHORS: F. A. Sebastião, L. R. Furlan, D. T. Hashimoto, F. Pilarski

  KEYWORDS: Direct Colony PCR, 16S rRNA Sequencing, Bacterial Fish Pathogens

  JOURNAL NAME: Advances in Microbiology, Vol.5 No.6, June 16, 2015

  ABSTRACT: Intensive fish farming systems in Brazil have increased the disease incidence, mainly of bacterial origin, due to higher stocking density, high organic matter levels and poor quality of the aquatic environment that causes high mortality rates during outbreaks. The identification of pathogenic species using a fast and reliable method of diagnosis is essential for successful epidemiological studies and disease control. The present study evaluated the use of direct colony PCR in combination with 16S rRNA gene sequencing to diagnose fish bacterial diseases, with the goal of reducing the costs and time necessary for bacterial identification. The method was successful for all 178 isolates tested and produced bands with the same intensity as the standard PCR performed using pure DNA. In conclusion, the genetics methods allowed detecting the most common and important pathogens in Aquaculture, including 12 species of occurrence in Brazilian fish farms. The results of the present study constitute an advance in the available diagnostic methods for bacterial pathogens in fish farms.