TITLE:
Predicting Adjuvant Chemotherapy Outcome by Simultaneous Analysis of Thymidylate Synthase Expression and p53 Nuclear Accumulation in Colorectal Cancer
AUTHORS:
Zihuan Yang, Dandan Huang, Lekun Fang, Xingzhi Feng, Huanliang Liu, Lei Wang, Jianping Wang
KEYWORDS:
Colorectal Cancer, p53, TS, 5-FU, Chemotherapy
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.6 No.5,
May
27,
2015
ABSTRACT: Studies have shown that the tumor
suppressor gene p53 may regulate thymidylate synthase (TS) activity in colorectal
cancer (CRC) cells, hence attributed to chemo-resistance to 5-flurouracil in
CRC. In this study, a total of 299 primary CRC patients who underwent surgery
alone or received an adjuvant 5-FU-based chemotherapy were retrospectively
studied. TS expression and p53 nuclear accumulation on paraffin embedded
primary tumor tissue arrays were immunohistochemically assessed, and their
relationship to patient overall survival (OS) and disease free survival (DFS)
were analyzed. No correlation was found between TS and p53 expression. p53
nuclear accumulation was significantly correlated with tumor location. In all,
multivariate analysis shows that TNM stage is a good indicator of patient
survival. TS or p53 is not an independent prognostic or predictive factor in the
CRCs. In chemotherapy-treated group, simultaneous analysis of TS and p53
indicates patients in the p53-/TS- or p53+/TS+ group have significant better OS
and DFS than the group p53-/TS+ or p53+/TS- (P