TITLE:
Determination of Salmonella pullorum with Nanoparticles Immune Based Lateral Flow Strip Assay
AUTHORS:
Bin Wu, Xiuyun Zhang, Wei Pan, Lin Zhang, Fan Zhang
KEYWORDS:
Monoclonal Antibody, Nanoparticles, Fluorescence Nanoparticles Immune Based Lateral Flow Strips, Salmonella pullorum
JOURNAL NAME:
Advances in Microbiology,
Vol.5 No.5,
May
25,
2015
ABSTRACT: The
isolation and culture of conventional detection method of salmonella can not meet the testing requirements of quick and easy
detection at the grassroots level. In this study, we prepare the fluorescent
nanoparticles as a marker, covalently conjugate with monoclonal antibodies of Salmonella pullorum. The whole Salmonella pullorum antigen and goat
anti-mouse antibody sprayed on the nitrocellulose membranes are used as test
line and control line. The fluorescence nanoparticles immune based lateral flow
strips are made according to the principle of antigen-antibody immune response.
The test strips may interpret results within 30 min. The results of the salmonella A, S. agona, S. chester and S. arechavaleta are positive,
including, S. agona for weakly
positive. After analysis, it is found that in addition to the salmonella of group A, the other
positive salmonella are in group B. But
it is negative of S. derby, S. rissen, and other 6 kinds of salmonella, with good specificity. The
fluorescence nanoparticles immune based lateral flow strips are a little of
sample can be detected fast, easily, inexpensive, easy to universal without
professional technical personnel detection method. It provides a new detection
method for the detections of Salmonella pullorum.