TITLE:
Lead-Induced Damage on Hepatocytes and Hepatic Reticular Fibres in Rats; Protective Role of Aqueous Extract of Moringa oleifera Leaves (Lam)
AUTHORS:
Bukola R. Omotoso, Adeleke A. Abiodun, Omamuyovwi M. Ijomone, Stephen O. Adewole
KEYWORDS:
Lead, Moringa oleifera, Liver, Reticular Fibres, Rats
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.3 No.5,
May
15,
2015
ABSTRACT: This study was designed to evaluate the hepatoprotective and ameliorative effects of aqueous extract of Moringa oleifera (MO) leaves on the histologyof liver and hepatic reticular fibres integrity of adult Wistar rats following lead-induced hepatotoxicity. Twenty four adult Wistar rats, weighing 180 - 220 g, randomly assigned into four groups of six animals each were used for the study. Lead and Moringa oleifera were given orally to the rats. 24 hours after the last administration, animals were sacrificed, blood obtained by cardiac puncture and liver excised, fixed in 10% phosphate buffered formalin for histological and histochemical analysis. The activities of aspartate amino transferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) were used as markers of hepatotoxicity and catalase (CAT) activity were used as marker to evaluate the anti-oxidant status of the tissue. Result showed that lead treatment increased markers of hepatic damage (ALT, AST and ALP) and decreased CAT activities. Histological studies reveal alterations of hepatic structure including hepatocytic vacuolations, sinuosoidal congestion and loss of reticular fibres following lead treatment. Treatment with MO prevented and reversed lead induced hepatic damage. In conclusion, this study shows that Moringa oleifera leaf extract has an appreciable ability to prevent hepatotoxicity caused by lead, partly as result of its chemical constituents which has hepatoprotective properties.