TITLE:
Production of Bioethanol at High Temperature from Tari
AUTHORS:
Ali Azam Talukder, Sirajul Islam Sujon, Muhammad Maqsud Hossain, Donald James Gomes, Mamoru Yamada
KEYWORDS:
Tari, Pantavat, Bioethanol, Thermotolerant Microorganisms, Fermentation
JOURNAL NAME:
Advances in Microbiology,
Vol.5 No.5,
May
14,
2015
ABSTRACT: Many
microorganisms can tolerate high-temperature ranges from 37°C - 45°C are called
thermotolerant microorganisms. Eighteen such isolates containing various
microorganisms were collected from the natural fermented products of Bangladesh
in summer for bioethanol production. Cultural, morphological, physiological,
biochemical and genetical analysis were carried out under various physiological
conditions. Among them, two thermotolerant strains Tari-6, isolated from the
Tari (an overnight natural fermented palm juice at around 33°C - 40°C) and
Pvt-1, isolated from the Pantavat (an overnight natural fermented rice soaked
with tap water at around 35°C - 37°C), produced high amount of bioethanol, 7.5%
(v/v) and 6.5% (v/v), respectively at 37°C. Furthermore, a partial 26S rDNA
sequencing results confirmed that the Tari-6 and Pvt-1 encoded Pichia
galeiformi and Pichia guilliermondii, respectively, and later one could grow well in media containing Xylose. Our results
conclude that these two yeast strains are the potential candidates for
bioethanol production.