TITLE:
Kainic Acid, NMDA and Bicuculline Induce Elevation in Concentrations of Glutathione and Amino Acids in Vivo: Biomarkers for Seizure Predisposition?
AUTHORS:
Abdul-Karim Abbas
KEYWORDS:
NMDA, Glutathione, Taurine, Hippocampus, Microdialysis, Kainic Acid, Seizure
JOURNAL NAME:
Journal of Behavioral and Brain Science,
Vol.5 No.5,
May
5,
2015
ABSTRACT: The present study
was carried out to investigate the effect of NMDA, bicuculline and kainic acid
(KA) on the extracellular concentration of glutathione, phosphoethanolamine
(PEA) and taurine in rat hippocampus in vivo. Rats were implanted with
intrahippocampal microelectrodes perfused with free-glucose Krebs-Ringer
solution and allowed to recover for about 2 h. After assaying baseline
concentrations of amino acids, NMDA or bicuculline was administered
intrahippocampally, whereas KA was given systemically. Either treatment
resulted in significant high extracellular concentrations of glutathione, but
only NMDA or KA resulted in high concentrations of PEA and taurine.
Interestingly, the increase in glutathione concentration due to KA was followed
by a delayed increase of glutamate and PEA. Our results demonstrated that
increased efflux of glutathione, a common consequence of different
neuroexcitotoxic agents, occurs in vivo.
Given that the agents used in the present study were also convulsunts, the
implication of the findings on seizure predisposition was also considered.