TITLE:
Tumor Cell-Extracellular Matrix Interaction Modulates MMP-1 in Breast Cancer
AUTHORS:
Sekhar Pal, Kirat Kumar Ganguly, Shyamsundar Mandal, Jaydip Biswas, Amitava Chatterjee
KEYWORDS:
α5β1 Integrin, Signaling, ECM, Fibronectin, MMP-1
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.6 No.4,
April
30,
2015
ABSTRACT: Increased MMP-1 expression in various tumor cells is significantly
correlated with cancer progression. Enhanced secreted and intracellular level
of MMP-1 is found in breast cancer cell line MDA-MB-231 in presence of ECM
glycoprotein fibronectin. To extrapolate this study into in vivo system,
we observed the expression of MMP-1, fibronectin and α5β1 integrin,
which is the receptor for fibronectin, in breast cancer tissue samples.
Expression of active form of MMP-1 was increased in tumor samples compared with
the non-tumor counterpart. In some samples pro-MMP-1 was decreased but active
form was increased in tumor part. The difference was more prominent in advanced
stage tumor. ELISA showed appreciable increase in expression of α5 and β1integrins
in tumor tissue in comparison to the non tumor counterpart in case of advanced
stage tumor. Though there is no appreciable difference in fibronectin
concentration, enhanced α5, β1 integrin
expression may mediate enhanced cell-ECM interaction to upregulate MMP-1 in
tumor samples compared with the matched control.