TITLE:
Interaction of Hemoglobin with Binuclearcationic Tetranitrosyl Iron Complex with Penicillamine. Cations Binding Sites
AUTHORS:
Lidia Syrtsova, Natalia Sanina, Boris Psikha, Ildar Tukhvatullin, Natal’ja Shkondina, Olesia Pokidova, Alexander Kotelnikov
KEYWORDS:
Biomedicine, Iron-Sulfur Cluster, Ligand Binding, Heme, Nitric Oxide
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.5 No.3,
April
28,
2015
ABSTRACT: In this paper, the kinetics of the interaction of the nitrosyl iron complex with the ligands penicillamine [Fe2(SC5H11NО2)2(NO)4]SO4·5H2O (I) with deoxyhemoglobin (Hb) was studied. The kinetic modeling method defined the number of binding (I) molecules and equilibrium constant of the coupling reaction of (Biomedicine, Iron-Sulfur Cluster, Ligand Binding, Heme, Nitric Oxide
) with Hb (Ks). At equimolar concentrations of (I) and Hb (2 × 10−5 M), the Hb molecule binds only one (I) with Ks equal to 4.3 × 107 M−1. When increasing the (Biomedicine, Iron-Sulfur Cluster, Ligand Binding, Heme, Nitric Oxide
) concentration, the number of binding sites of Hb increases and Ks decreases. These results are analyzed in accordance with the data on the existence of cations binding sites in Hb.