TITLE:
Phenotypic Characterization Murine Sarcoma TG-180 Immunophenotypical Characterization Murine Sarcoma TG-180
AUTHORS:
Thais M. T. Bernardes, Hugo E. O. Beserra, Stephane C. O. R. Vexenat, Helio Langoni, Noeme S. Rocha
KEYWORDS:
Immunohistochemistry, Neoplasia, Mice, Tumor Origin
JOURNAL NAME:
Open Journal of Pathology,
Vol.5 No.2,
April
24,
2015
ABSTRACT: The sarcoma is the generic nomenclature for
neoplasm of mesodermal cells, which express in man and animals. Silent growth
requires early diagnosis technique for identifying their proteins. The
experimental model in vivo murine sarcoma 180-TG (TG-180), is widely used in
research to provide the stimuli of infectious and neoplastic antigens. In this
case, the technique of immunohisto-chemistry helps identify the expressions of
tumor cell variants. The objective of the research was to characterize
immunoexpression murine sarcoma TG 180, by immunohistochemistry, antibodies
AE1/AE3, vimentin, CD3, CD 45, CD79α and S100A4. For this, murine sarcoma
TG-180, was implanted subcutaneously in 20 mice “Swiss”, male, 30 days old, 28
g for 10 days. Samples were taken and subjected to immunohistochemistry, via
use of HistoMouse-MA™? kit. There was specifically labeled S100A4 and vimentin
antibodies, indicative of poorly differentiated neoplasms fibroblasts. In
fact, the model is established by identifying the origin of the cell, once
identified, chemotherapeutic tests can also be performed. Neoplasia like these,
when installed in man and animals, depending on the degree of aggressiveness
requires treatment protocol varied between surgery and chemotherapy or
combination of treatments.