TITLE:
Antioxidant, Enzyme Inhibitory and Anti-Obesity Potential of Sorrel Calyx Extracts in 3T3-L1 Adipocytes
AUTHORS:
E. Mutai, R. Sunkara, Jorge Vizcarra, L. T. Walker, M. Verghese
KEYWORDS:
Sorrel, Phytochemicals, Adipogenesis, Adipocytes, Lipolysis, Antioxidant
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.6 No.5,
April
1,
2015
ABSTRACT: Hibiscus sabdariffa L. (sorrel) has been widely used in the
development of tropical beverages and folk medicine. This study’s objective was
to investigate the anti-obesity potential of sorrel calyx extracts (methanol
and water) on 3T3-L1 adipocyte cells. Phytochemical content, antioxidant potential
as DPPH (1, 1-Diphenyl-2-picrylhydrazyl) radical scavenging
activity and ferric reducing antioxidant power (FRAP) and enzyme (α-glucosidase, α-amylase, and pancreatic lipase) inhibitory activities were
determined in sorrel methanol extracts (SME) and sorrel water extracts (SWE).
Effect of SWE and SME on lipid accumulation, lipolysis and apoptosis were
tested in 3T3-L1 adipocyte differentiation and maintenance stage of cells at
selected concentrations (200 - 1000 μg/ml) was studied. The total phenolic (GAE
mg/100g dry weight) and total flavonoid (mg catechin equi/ 100g dry weight) contents in SME and SWE were 158.31 and 317.27
and 90.77 and 100.08. DPPH% inhibition
(IC-50-mg/ml) and FRAP (mmol Fe [II]/100g dry weight) were 0.82 and
0.33 and 1799.13 and 2296.38 for SWE and SME, respectively. SME and SWE
inhibited α-glucosidase, α-amylase, and pancreatic lipase
activities by more than 40% at 4mg/ml. Significant (p