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Fau, D., Berson, A., Eugene, D., Fromentry, B., Fisch, C. and Pessayre D. (1992) Mechanism for the Hepatotoxicity of the Antiandrogen Nilutamide. Evidence Suggesting that Redox Cycling of this Nitroaromatic Drug Leads to Oxidative Stress in Isolated Hepatocytes. Journal of Pharmacology and Experimental Therapeutics, 263, 69-77.

has been cited by the following article:

• TITLE: Protective Effects of Saponin Mixture, Isolated from Astragalus monspessulanus subsp. monspessulanus on Tert-Butyl Hydroperoxide—Induced Oxidative Stress in Isolated Rat Hepatocytes

  AUTHORS: Magdalena Spasova Kondeva-Burdina, Viktor Bratkov, Rumyana Lubomirova Simeonova, Vessela Bisserova Vitcheva, Ilina Nikolaeva Krasteva, Petranka Krumova Zdraveva

  KEYWORDS: Astragalus monspessulanus, Saponins, Hepatocytes, Antioxidant Activity, Cytoprotection

  JOURNAL NAME: American Journal of Plant Sciences, Vol.6 No.6, March 31, 2015

  ABSTRACT: Saponin mixture, obtained from Astragalus monspessulanus subsp. monspessulanus (Fabaceae) was investigated for possible protective effect on tert-butyl hydroperoxide (t-BuOOH)-induced cytotoxicity using primary isolated rat hepatocytes. The cells were isolated by two-stepped col-lagenase perfusion. Liver damage was induced by one hour incubation with t-BuOOH (75 μmol·L-1) and discerned by decreased cell viability, increased lactate dehydrogenase (LDH) leakage into the medium, increased production of malondialdehyde (MDA) and depletion of the cell protector glutathione (GSH). Cell pre-incubation with the saponin mixture (1 mg/mL and 5 mg/mL) significantly (p