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Council of Europe (1991) European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes. CETS No. 123.
http://conventions.coe.int/Treaty/Commun/QueVoulezVous.asp?NT=123&CM=1&CL=ENG

has been cited by the following article:

• TITLE: Protective Effects of Saponin Mixture, Isolated from Astragalus monspessulanus subsp. monspessulanus on Tert-Butyl Hydroperoxide—Induced Oxidative Stress in Isolated Rat Hepatocytes

  AUTHORS: Magdalena Spasova Kondeva-Burdina, Viktor Bratkov, Rumyana Lubomirova Simeonova, Vessela Bisserova Vitcheva, Ilina Nikolaeva Krasteva, Petranka Krumova Zdraveva

  KEYWORDS: Astragalus monspessulanus, Saponins, Hepatocytes, Antioxidant Activity, Cytoprotection

  JOURNAL NAME: American Journal of Plant Sciences, Vol.6 No.6, March 31, 2015

  ABSTRACT: Saponin mixture, obtained from Astragalus monspessulanus subsp. monspessulanus (Fabaceae) was investigated for possible protective effect on tert-butyl hydroperoxide (t-BuOOH)-induced cytotoxicity using primary isolated rat hepatocytes. The cells were isolated by two-stepped col-lagenase perfusion. Liver damage was induced by one hour incubation with t-BuOOH (75 μmol·L-1) and discerned by decreased cell viability, increased lactate dehydrogenase (LDH) leakage into the medium, increased production of malondialdehyde (MDA) and depletion of the cell protector glutathione (GSH). Cell pre-incubation with the saponin mixture (1 mg/mL and 5 mg/mL) significantly (p