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Wang, D., Shen, W., Tian, Y., Sun, Z., Jiang, C. and Yuan, S. (1996) Protective Effect of Active Components Extracted from Radix Astragali on Human Erythrocyte Membrane Damages Caused by Reactive Oxygen Species. China Journal of Chinese Materia Medica, 21, 746-763.

has been cited by the following article:

• TITLE: Protective Effects of Saponin Mixture, Isolated from Astragalus monspessulanus subsp. monspessulanus on Tert-Butyl Hydroperoxide—Induced Oxidative Stress in Isolated Rat Hepatocytes

  AUTHORS: Magdalena Spasova Kondeva-Burdina, Viktor Bratkov, Rumyana Lubomirova Simeonova, Vessela Bisserova Vitcheva, Ilina Nikolaeva Krasteva, Petranka Krumova Zdraveva

  KEYWORDS: Astragalus monspessulanus, Saponins, Hepatocytes, Antioxidant Activity, Cytoprotection

  JOURNAL NAME: American Journal of Plant Sciences, Vol.6 No.6, March 31, 2015

  ABSTRACT: Saponin mixture, obtained from Astragalus monspessulanus subsp. monspessulanus (Fabaceae) was investigated for possible protective effect on tert-butyl hydroperoxide (t-BuOOH)-induced cytotoxicity using primary isolated rat hepatocytes. The cells were isolated by two-stepped col-lagenase perfusion. Liver damage was induced by one hour incubation with t-BuOOH (75 μmol·L-1) and discerned by decreased cell viability, increased lactate dehydrogenase (LDH) leakage into the medium, increased production of malondialdehyde (MDA) and depletion of the cell protector glutathione (GSH). Cell pre-incubation with the saponin mixture (1 mg/mL and 5 mg/mL) significantly (p