TITLE:
Protective Effects of Saponin Mixture, Isolated from Astragalus monspessulanus subsp. monspessulanus on Tert-Butyl Hydroperoxide—Induced Oxidative Stress in Isolated Rat Hepatocytes
AUTHORS:
Magdalena Spasova Kondeva-Burdina, Viktor Bratkov, Rumyana Lubomirova Simeonova, Vessela Bisserova Vitcheva, Ilina Nikolaeva Krasteva, Petranka Krumova Zdraveva
KEYWORDS:
Astragalus monspessulanus, Saponins, Hepatocytes, Antioxidant Activity, Cytoprotection
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.6 No.6,
March
31,
2015
ABSTRACT: Saponin mixture, obtained from Astragalus monspessulanus subsp. monspessulanus (Fabaceae) was investigated for possible protective effect on tert-butyl hydroperoxide (t-BuOOH)-induced cytotoxicity using primary isolated rat hepatocytes. The cells were isolated by two-stepped col-lagenase perfusion. Liver damage was induced by one hour incubation with t-BuOOH (75 μmol·L-1) and discerned by decreased cell viability, increased lactate dehydrogenase (LDH) leakage into the medium, increased production of malondialdehyde (MDA) and depletion of the cell protector glutathione (GSH). Cell pre-incubation with the saponin mixture (1 mg/mL and 5 mg/mL) significantly (p