TITLE:
Evaluating the p-FOXO1/FOXO1 Ratio: An Alternative Strategy for Endometrial Cancer Diagnosis
AUTHORS:
Mohsen Korani, Soudabeh Fallah
KEYWORDS:
Endometrial Cancer, FOXO1, p-FOXO1, Transcription Factor
JOURNAL NAME:
International Journal of Clinical Medicine,
Vol.6 No.3,
March
26,
2015
ABSTRACT: Background: The FOXO subfamily of Forkhead transcription factors plays a central role in pro-moting expression of proapoptotic and cell cycle regulatory genes. FOXO1 expression has an im-portant role in human endometrium homeostasis. Therefore, reduced FOXO1 protein and its inactivation by phosphorylation might, play a role in progression of human endometrial cancer. Methods: Current study was designed to investigate the changes of the FOXO1, phosphorylated-FOXO1 (p-FOX1) proteins levels and the p-FOXO1/FOXO1 ratio in 30 patients with endometrial cancer and 20 subjects with normal endometrium, surgically using excised human endometrial tissue specimens, quantitative real time PCR and western blot methods. Results: FOXO1 protein level in patients with endometrial cancer significantly reduced in comparison with control group (0.17 ± 0.15 vs. 1 ± 0.14; p