TITLE:
Screening and Identification of Chitinolytic Actinomycetes and Study on the Inhibitory Activity against Turfgrass Root Rot Disease Fungi
AUTHORS:
Qi Wang, Bihua Duan, Bihua Duan, Rui Yang, Yaorong Zhao, Lu Zhang
KEYWORDS:
Strain Bn035, Transparent Circle, Chitinase Activity, Antagonism, 16SrDNA
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.3 No.3,
March
18,
2015
ABSTRACT: Objective: This study aimed to separate and
pure chitinase producing actinomycetes with stronger activity from lawn soils
of Beijing. Method: Morphology and culture characteristics were observed. physiological
and biochemical characteristics were detected. 16SrDNA sequence was analyzed. Result:
The highest chitinase activity strain was Bn035, whose relative diameter of transparent
circle K2 was 3.42 at which the chitinase activity of fermentation broth reached
a maximum value of 1.23 U/mL in the 6th day. Crude enzyme solution and bacteria
cake of strain Bn035 generated stronger bacterial resistance to all root rot
fungi which indicated that the strain had a strong broad-spectrum resistance to
root rot fungi. Conclusion: The strain was identified as Streptomyces
scabrisporus. It will be a potential biological control function.