TITLE:
Mec1-Dependent Phosphorylation of the Scc3 Subunit of Cohesin during Mitosis in Budding Yeast
AUTHORS:
Nathalie Grandin, Michel Charbonneau
KEYWORDS:
Cohesin, S/T-Q Phosphorylation, Mec1 and Tel1, DNA Damage, Cell Cycle, Budding Yeast
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.6 No.3,
March
17,
2015
ABSTRACT: Cohesin is an evolutionary conserved complex that controls chromosome segregation during mitosis. Here we show that, in response to DNA damage, Saccharomyces cerevisiae Scc3, one of the major regulatory subunits of the Smc1-Smc3-Scc1 cohesin ring, is phosphorylated on S/T-Q residues. This event depended on the Mec1 checkpoint kinase as well as on cell cycle arrest triggered by the DNA damage checkpoint network. This phosphorylation event also took place during mitosis of an unperturbed cell cycle. The present finding that S. cerevisiae Scc3 is phosphorylated during mitosis represents a potentially important new regulatory step in cohesin’s mitotic functions.