TITLE:
Antioxidant, Collagen Synthesis Activity in Vitro and Clinical Test on Anti-Wrinkle Activity of Formulated Cream Containing Veronica officinalis Extract
AUTHORS:
Ha Youn Lee, Amal Kumar Ghimeray, Jun Hwan Yim, Moon Sik Chang
KEYWORDS:
Antioxidant, Antiwrinkle, Collagen Synthesis, Veronica officinalis, Scoti-SpeedwellTM
JOURNAL NAME:
Journal of Cosmetics, Dermatological Sciences and Applications,
Vol.5 No.1,
March
17,
2015
ABSTRACT: In this study, our objective was to evaluate the antioxidant,
cytotoxicity and collagen synthesis activity in vitro and also to test the anti-wrinkle effect of formulated
cream containing Veronica officinalis extract in vivo. Antioxidant evaluation was
based on the scavenging activity of free radicles (DPPH) and procollagen type 1
protein (P1P) synthesis test was performed in fibroblast cell. Clinical
anti-wrinkle activity was performed on female subjects in placebo-controlled
trail. Verbascoside (an isolated compound) showed higher (IC50 value
of 36.24 ± 1.81 μg/ml) free radicle inhibition activity but weaker collagen
synthesis activity. The ethanolic extract showed good inhibition to DPPH free
radicals and also showed a significant effect in collagen synthesis activity
without cytotoxicity. In the in
vivo study, treatment with the formulated cream (Scoti-Speedwell) for 56
days significantly reduced the percentage of wrinkle area and length with 18.0%
and 16.05%, respectively. Overall, Veronica
officinalis extract containing product (Scoti-SpeedwellTM) can be regarded
as a potent anti-wrinkle agent in human skin.