TITLE:
Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker
AUTHORS:
Letícia Sayuri Murate, Admilton Gonçalves de Oliveira, Allan Yukio Higashi, André Riedi Barazetti, Ane Stefano Simionato, Caroline Santos da Silva, Glenda Cavalari Simões, Igor Matheus Oliveira dos Santos, Marlon Renan Ferreira, Martha Viviana Torres Cely, Miguel Octavio Pérez Navarro, Vanessa Fogaça de Freitas, Marco Antonio Nogueira, João Carlos Palazzo de Mello, Rui Pereira Leite Jr., Galdino Andrade
KEYWORDS:
X. axonopodis pv. citri, Citrus sinensis, Antibiosis, Biological Control, Natural Antibiotic
JOURNAL NAME:
Agricultural Sciences,
Vol.6 No.3,
March
10,
2015
ABSTRACT: This study
investigated the protective effects of secondary bacterial metabolites,
produced by Pseudomonas sp.
(bacterium strain LN), on citrus canker disease caused by Xanthomonas axonopodis pv. citri (Xac 306). The LN bacteria strain was
cultured in liquid medium and the supernatant free-cells was treated with
methanol (AMF) and ethyl acetate (AEF), respectively, and then the extract was
concentrated, filtrated, lyophilized and fractionated by vacuum liquid
chromatography (VLC). After VLC, eight fractions were obtained. All fractions’
activity against Xac 306 by agar well diffusion assay and minimum inhibitory
concentration but in different concentrationswere tested. Cytotoxicity effects
were observed in all fractions in 50 μg·mL-1 concentration. The
comet assay demonstrated that the fractions EAF, VLC2 and VLC3 presented no
genotoxic effects at tested concentrations. In plants only VLC3 showed
significant results (p