TITLE:
A Peptide Nucleic Acid to Reduce Type I Collagen Production by Fibroblast Cells
AUTHORS:
Yasutada Imamura, Suzuka Tsuboi, Toru Sugiyama, Atsushi Kittaka, Yonchol Shin
KEYWORDS:
Collagen, PNA, Antigene, Fibrosis, Vimentin
JOURNAL NAME:
Open Journal of Medicinal Chemistry,
Vol.5 No.1,
March
6,
2015
ABSTRACT: In this study, we prepared a peptide nucleic acid (PNA) against the gene coding for the human alpha 1 chain of type I collagen. This PNA was incorporated into normal human fibroblast cells by electroporation, leading to a decrease in the mRNA level of the gene. Furthermore, mRNA for the alpha 2 chain of type I collagen was also reduced. The production of collagen protein exhibited a similar profile to the changes in mRNA. These results indicate that PNA targeting COL1A1 is effective as an antigene reagent, and opens the possibility of future clinical applications in fibroproliferative disorders.