TITLE:
Therapeutic Potential of 17β Estradiol with Tachykinin Neuropeptide NKB and Aβ(25 - 35) on Na+ - K+ ATPase Activity in Aging Female Rat Brain
AUTHORS:
Rashmi Jha, Priyanka Mishra, Ranjeet Kumar, Abbas Ali Mahdi, Shivani Pandey, Najma Zaheer Baquer, Sudha Mahajan Cowsik
KEYWORDS:
Na+ - K+ ATPase, Aging, Neurokinin B, Amyloid Beta (25 - 35), Estradiol
JOURNAL NAME:
Advances in Aging Research,
Vol.4 No.2,
February
26,
2015
ABSTRACT: The Na+ - K+ ATPase is an
enzyme responsible for the active transport of Na+ and K+ in most eukaryotic cells. The aim of the present study was to determine the
effect of Tachykinin neuropeptide, Neurokinin B (NKB) and Amyloid beta fragment
Aβ (25 - 35) on 17β estradiol (E2) treated aging female rat brain synaptosomes of
different age groups, by assaying Na+ - K+ ATPase enzyme
activity. An in vitro incubation of
isolated synaptosomes with Aβ (25 - 35)
showed toxic effects while NKB showed stimulating effect on the Na+ - K+ ATPase activity, and the combined NKB + Aβ (25 - 35) incubations showed a partial effect
as compared to the Aβ (25 - 35)
alone. To understand whether E2 affects the expression of Na+ - K+ ATPase
molecules, we examined the expression of Na+ - K+ ATPase
subunit α1 and β2 in E2 treated aging female rat brain synaptosomes. The enzyme
was quantified by SDS PAGE in control and E2 treated rat brain. We observed
that the expression of α1 and β2 Na+ - K+ ATPase
molecules increased and reversed to a normal level in E2 treated synaptosomes.
These results confirmed that E2 increased turnover of Na+ - K+ ATPase molecules in aging rat brain. The
present findings also suggest a possible role of NKB with E2 in the age related changes in the brain.