TITLE:
Development of Preparative Chromatography for Proteomic Approach of Mycorrhizal Symbiosis
AUTHORS:
Tomoko Matsubara, Takaaki Ishii
KEYWORDS:
24 kDa Protein, Mycorrhizal Fungi, Preparative Chromatography, Proteomic Analysis, Symbiosis
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.5 No.1,
February
2,
2015
ABSTRACT: Although mechanism of symbiosis between arbuscular mycorrhizal fungi (AMF) and host plants has been investigated by genetic analysis, very little knowledge has been obtained because genome analysis of AMF is not perfect yet. Thus, we tried to develop mass purification of proteins using preparative chromatography in order to accelerate roteomic analysis of proteins related to mycorrhizal symbiosis, such as 24 and 53 kDa proteins. In particular, our data showed that 53 kDa proteins would be restrictively expressed when mycorrhizal fungi and host plants were stressed. However, 24 kDa proteins, which appear to be a usable indicator for the existence of various my-corrhizal fungi, were habitually detected in not only AMF but also other mycorrhizal fungi such as ectomycorrhizal fungi (EF). Moreover, we discovered new preparative chromatographical techniques for isolation and mass purification of those proteins. We are convinced that this chromato-graphical technique will greatly contribute to proteomic approach of mycorrhizal symbiosis.