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Selvaraj, N., Vengadesan, G., Vasudevan, A., Prem, A.R., Ramesh, A.V. and Ganapathi, A. (2002) Micropropagation of Cucumis sativus L. from Field Grown Plants. In: Maynard, D.N., Ed., Proceedings of the Cucurbitaceae, ISHS Press, Belgium, 149-156.

has been cited by the following article:

• TITLE: Direct Organogenesis from Cotyledonary Node Explants of Cucurbita pepo (L.)—An Important Zucchini Type Vegetable Crop

  AUTHORS: Muruganantham Mookkan

  KEYWORDS: Cucurbita pepo, Zucchini, Cotyledonary Node, Subculture

  JOURNAL NAME: American Journal of Plant Sciences, Vol.6 No.1, January 15, 2015

  ABSTRACT: Cotyledonary node explants from 3 - 5-d-old seedling derived explants of Cucurbita pepo (L.) produced multiple shoots in Murashige and Skoog (MS) salts B5 vitamins containing medium in the presence of N6-benzylamino-purine BAP 1 mg/L + Thidiazuron TDZ 0.05 mg/L. After 4 weeks explants were subcultured to medium containing MS salts B5 Vitamins BAP 0.5 mg/L, Gibberellic acid GA3 1 mg/L and L-Glutamine 15 mg/L. Periodic excision of regenerated shoots from the explants increased shoot efficiency during subculture. The combination of L-Glutamine with BA and GA3 significantly increased the shoot proliferation. Elongated shoots were excised from shoot clumps and transferred to rooting medium containing indole-3-butyric acid (IBA, 1.0 mg/L). The rooted plants were hardened in small pot containing standardized garden soil, well developed plant transferred to greenhouse and survival rate was 85%.