TITLE:
An Insight on Small Molecule Induced Foot-Print Free Naive Pluripotent Stem Cells in Livestock
AUTHORS:
Mengmei Li, Lanyu Li, Jun Zhang, Vinod Verma, Qingyou Liu, Deshun Shi, Ben Huang
KEYWORDS:
Small Molecule, Foot-Print Free, Naive Pluripotent Stem Cell, Livestock
JOURNAL NAME:
Stem Cell Discovery,
Vol.5 No.1,
January
7,
2015
ABSTRACT: Bona fide embryonic stem cell (ESC) lines from livestock species have been challenging to derive and maintain, contrasting mouse and human ESCs. However, induced pluripotent stem cells (iPSC) generated by reprogramming somatic cells tender an option, as they display characteristic features of ESC. The comprehension that induced pluripotent stem cells (iPSC) could be created with in no time also holds the potential of allowing pluripotent cells to be derived from animal models vital in biomedical research. Endeavors to produce bona fide pluripotent stem cells (PSC) from livestock have been going on for more than two decades. But, attempts to derive bona fide livestock iPS cells have met with limited success. Recently it’s been reported that small molecules can augment reprogramming efficiency and may be used to substitute few or all transcription factors used for reprogramming. It is assumed that the reprogramming factors are conserved among species, and this small molecule reprogramming approach will probably apply to livestock species as well. So this review will focus mainly on the accomplishments of small molecules on accelerating cell reprogramming and obtaining naive pluripotency, and raise a new insight on, exogenous genes free, livestock naive iPSC generation with a new bullet, small molecule.