TITLE:
Expression of Messenger RNA of Oncoproteins E6 and E7 of Human Papilomavirus in Women with Negative Oncotic Cytologies, Epithelial Squamous Atypias of Undefined Significance and Low-Grade Intraepithelial Lesions
AUTHORS:
Daiene Carvalho Casagrande, Julisa Chamorro Lascasas Ribalta, Katia de Araujo Prado Ferreira Leite, Manoel Schimidt, Neila Maria de Góis Speck
KEYWORDS:
E6/E7 RNAm, Human Papillomavirus, Negative Cytology, ASC-US Cytology, LSIL Cytology
JOURNAL NAME:
Open Journal of Obstetrics and Gynecology,
Vol.5 No.1,
January
6,
2015
ABSTRACT: Objectives: To verify the prevalence of E6/E7 RNAm expression of
HPV in patients with negative cervicovaginal cytology, ASC-US and LSIL; to
correlate with negative anatomopathological exams and/or squamous
intraepithelial neoplasy grade I (SIN 1) of the lower genital tract (LGT); to
relate the RNAm expression with viral infection types; to assess the genotyping
in single infections. Methods: Findings from 825 women submitted to E6/E7 RNAm
survey and 422 women submitted to LGT biopsies were analyzed. Results: A larger
percentage of E6/E7 in ASC-US and LSIL cytologies occurred. Negative results of
RNAm expression were in accordance with negative cytologies and negative
anatomopathological exam. In positive cases, the infection by a single HPV type
was most prevalent, with the type 16 being the most common. Conclusions: the
expression of mRNA was most prevalent in ASC-US and LSIL cytologies, comparing
with the negative ones. The findings of SIN 1 biopsies were related to the
positive expression of mRNA and negative cytologies; the negative expression
was in agreement with negative anatomopathological exam. The infection by a HPV
type was more frequent in cases of positive expression, the HPV type 16 being
most frequently found. Patients with low grade intraepithelial lesion
cytologies had a higher percentage of multiple infections.