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Sugimoto, T., Sueyoshi, K. and Oji, Y. (1997) Increase of PEPC Activity in Developing Rice Seeds with Nitrogen Application at Flowering Stage. In: Ando, T., Fujita, K., Mae, T., Matsumoto, H., Mori, S. and Sekiya, J., Eds., Plant Nutrition for Sustainable Food Production and Environment—Proceedings of the 13th International Plant Nutrition Colloquium, Tokyo, 13-19 September 1997, Springer, Netherlands, 811-812.

has been cited by the following article:

• TITLE: Concomitant Increases of the Developing Seed Phosphoenolpyruvate Carboxylase Ac-tivity and the Seed Protein Content of Field-Grown Wheat with Nitrogen Supply

  AUTHORS: Naoki Yamamoto, Toshio Sugimoto, Takehiro Masumura

  KEYWORDS: Nitrogen Application, Phosphoenolpyruvate Carboxylase, Seed Storage Protein, Wheat

  JOURNAL NAME: Agricultural Sciences, Vol.5 No.14, December 29, 2014

  ABSTRACT: Wheat seed storage protein is of great importance for human food. To increase the contents of storage proteins effectively, nitrogen fertilizer at flowering stages is commonly applied. In our previous study, rice phosphoenolpyruvate carboxylase (PEPCase) activity in developing seeds was observed in response to nitrogen application at a flowering stage and was positively correlated to the response of the protein content in seeds of six cultivars. This observation might indicate that the seeds have a biological system for accepting nitrogen in seeds by using PEPCase. To test whether this physiological event occurs in wheat, we examined the PEPCase activity and protein content in field-grown wheat seeds under different nitrogen supply conditions. With only basal dressing, seeds showed lower PEPCase activity and protein content (both 0.90-fold) compared to seeds without basal fertilizer. With ammonium sulfate application at 8.3 and 25 g/m2 at a flowering stage, seeds showed higher PEPCase activity (1.08- and 1.17-fold, respectively) and protein content (1.15- and 1.42-fold, respectively), depending on the nitrogen level. We investigated the relationship between PEPCase activity and protein content in the seeds among four conditions. The effect of the nitrogen supply on PEPCase activity during grain-filling stages was validated by the results of a hydroponic culture experiment. Together the results demonstrate that our hypothesis seems to apply to field-grown wheat.