TITLE:
Experimental Validation and Simulation of Fourier and Non-Fourier Heat Transfer Equation during Laser Nano-Phototherapy of Lung Cancer Cells: An in Vitro Assay
AUTHORS:
Mohammad E. Khosroshahi, Lida Ghazanfari, Payam Khoshkenar
KEYWORDS:
Bioheat Equation, Gold Nanoshells, Laser Hyperthermia, Lung Cancer Cells, Surface Plasmon Resonance
JOURNAL NAME:
Journal of Modern Physics,
Vol.5 No.18,
December
29,
2014
ABSTRACT: This paper investigated the numerical scheme extended to solve the hyperbolic
non-Fourier form of bioheat transfer equation and the experimental trials were
conducted to validate the numerical simulation. MNPs were prepared via
co-precipitation and modified with a silica layer. The amino modified Fe3O4/SiO2 nanoshells were covered with gold colloids producing nanoshells of Fe3O4/SiO2/Au
(MNSs). In vitro assays were
performed to determine the effect of apoptosis of QU-DB lung cancer cells based
on the cells morphology changes. Cell damage was reduced by decreasing the
power density of laser. Also, a larger area of damage on cell culture plates
was observed at longer intervals of laser irradiation. The effect of nanoshell
concentration and irradiation rate has been evaluated. A maximum temperature
rise of 6°C was achieved at 184 W/cm2 and concentration of 0.01
mg/ml. The experiment confirmed a hyperbolic behaviour of thermal propagation.
The results revealed that the three-dimensional implementation of bioheat equation
is likely to be more accurate than the two-dimensional study.