TITLE:
The Association between Mutations Detected in Tissue and Plasma from Patients with Colorectal Adenoma and Adenocarcinoma
AUTHORS:
Caroline Brenner Larsen, Jan Lindebjerg, Rikke Fredslund Andersen, Niels Pallisgaard, Susanne Holck, Anders Jakobsen, Hans Jørgen Nielsen
KEYWORDS:
KRAS, BRAF, Adenomas, Adenocarcinomas, Carcinogenic Development
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.5 No.14,
December
15,
2014
ABSTRACT: Most colorectal cancers
evolve through an adenoma-carcinoma sequence with mutations in the KRAS/BRAF
pathway as an early event. Mutation analyses are usually performed on tissue
samples, but during the last couple of years the same analysis in blood has
been facilitated. Our aim was to investigate the correlation between BRAF/KRAS
mutations in tissue and plasma from colorectal adenomas and adenocarcinomas.
Out of 22 patients with adenomas 10 had a mutation in the tissue, but no
mutations were detected in the plasma. In 10 of 26 adenocarcinomas a mutation
was found in the tumor and in four of these, the mutation was also detected in
the plasma. Our results confirm previous findings that mutated DNA in plasma
can be detected in approximately 50%of
non-metastasized adenocarcinomas. The difference between adenomas and
adenocarcinomas suggests that appearance of mutated DNA in plasma associates
with invasion.