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Hansen, H.H., Ebbesen, C., Mathiesen, C., Weikop, P., Ronn, L.C., Waroux, O., Scuvee-Moreau, J., Seutin, V. and Mikkelsen, J.D. (2006) The KCNQ Channel Opener Retigabine Inhibits the Activity of Mesencephalic Dopaminergic Systems of the Rat. Journal of Pharmacology and Experimental Therapeutics, 318, 1006-1019.
http://dx.doi.org/10.1124/jpet.106.106757

has been cited by the following article:

• TITLE: The Effects of Zinc and Other Divalent Cations on M-Current in Ventral Tegmental Area Dopamine Neurons

  AUTHORS: Susumu Koyama, Munechika Enjoji, Mark S. Brodie, Sarah B. Appel

  KEYWORDS: Divalent Cation, Dopaminergic, Nystatin-Perforated Patch Recording, Zinc

  JOURNAL NAME: Journal of Biomedical Science and Engineering, Vol.7 No.14, December 15, 2014

  ABSTRACT: Ventral tegmental area dopamine (DA VTA) neurons are important for the reinforcing effects of drugs of abuse such as ethanol and nicotine. We have previously shown that M-current (IM) regulates the excitability of DA VTA neurons. Zinc (Zn2+) contributes to the regulation of neuronal excitation as a neuromodulator. In the present study, we investigated zinc effect on the properties of IM and the spontaneous firing frequency of DA VTA neurons. The standard deactivation protocol was used to measure IM during voltage-clamp recording with a hyperpolarizing voltage step to ﹣40 mV from a holding potential (VH) of ﹣25 mV. Zn2+ (100 μM) inhibited IM amplitude and IM recovered completely from the inhibition after the washout of Zn2+. Zn2+ inhibited IM in a concentration-dependent manner (IC50: 5.8 μM). When hyperpolarizing voltage steps were given to ﹣65 mV (in 10 mV increments) from a VH of ﹣25 mV, Zn2+ (100 μM) reduced IM amplitude at each voltage and zinc inhibition of IM was not voltage-dependent. Zn2+ increased the spontaneous firing frequency of DA VTA neurons in a concentration-dependent manner, suggesting that Zn2+ causes excitation of DA VTA neurons through an action on IM. IM of DA VTA neurons was inhibited by 100 μM divalent cations in increasing order of potency: Ba2+ (16%) 2+ (25%) 2+ (40%) 2+ (59%) 2+ (67%). These results suggest that Zn2+ may exert physiologically significant regulation of neuronal excitability in DA VTA neurons.