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Dirikolu, L., Woods, W.E., Boyles, J., Lehner, A.J., Harkins, J.D., Fisher, M., Schaeffer, D.J. and Tobin, T. (2009) Nonsteroidal Anti-Inflammatory Agents and Musculoskeletal Injuries in Thoroughbred Racehorses in Kentucky. Journal of Veterinary Pharmacology and Therapeutics, 32, 271-279.
http://dx.doi.org/10.1111/j.1365-2885.2008.01038.x

has been cited by the following article:

• TITLE: Doping Control Analysis of 16 Non-Steroidal Anti-Inflammatory Drugs in Equine Plasma Using Liquid Chromatography-Tandem Mass Spectrometry

  AUTHORS: Youwen You, Cornelius E. Uboh, Fuyu Guan, Lawrence R. Soma

  KEYWORDS: Equine Plasma, Doping Control, Non-Steroidal Anti-Inflammatory Drugs, Liquid

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.5 No.17, December 11, 2014

  ABSTRACT: Non-steroidal anti-inflammatory drugs (NSAIDs) are classified as Class 4 agents by the Association of Racing Commissioners International and are banned in racehorses during competition in Pennsylvania (PA). To control the abuse of these agents in racehorses competing in PA, a forensic method for screening and confirmation of the presence of these agents is needed. Equine plasma (0.5 mL) was acidified with 75 μL 1M H3PO4 to increase recovery of the analytes by liquid-liquid extraction using methyl tert-butyl ether (MTBE). Extracted analytes were separated by reversed-phase liquid chromatography using a C8 column under gradient condition. All 16 analytes were detected, quantified and confirmed using a triple quadrupole tandem mass spectrometry with selected reaction monitoring (SRM) in both negative and positive electrospray ionization modes. The limit of detection, quantification and confirmation of the analytes were 1.0 - 5.0 ng/mL, 1.0 - 5.0 ng/mL and 1.0 - 20 ng/mL, respectively. The linear dynamic range of quantification was 5.0 - 200 ng/mL. The method is routinely used in anti-doping analysis to control the abuse of NSAIDs in racehorses competing in PA.