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Singh, M. N., Stringfellow, H. E., Walsh, M. J., Ashton, K. M., Paraskevaidis, E., Abdo, K. R., et al, (2008) Quantifiable mRNA transcripts for tamoxifen-metabolising enzymes in hu-man endometrium, Toxicology, Limerick, 249, 85–90.

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• TITLE: MRTF-A transactivates COMT gene and decreases the anti-tumor effects of tamoxifen

  AUTHORS: Zhi-Peng Liu, Xue-Gang Luo, Shu Guo, Jian-Xin Wang, Xin Zhang, Nan Wang, Yong Jiang, Tong-Cun Zhang

  KEYWORDS: MRTF-A; Tamoxifen; COMT; Breast Cancer

  JOURNAL NAME: Journal of Biomedical Science and Engineering, Vol.2 No.7, November 20, 2009

  ABSTRACT: Myocardin-related transcription factors A (MRTF-A) is a myocardin-related transcription factor that have been found strongly activated CarG box–containing genes through its direct binding to serum response factor (SRF). In the present study, the MRTF-A ex-pression vector was constructed. The MTT assay showed that transfection of MRTF-A could significantly decrease the anti-tumor effect of tamoxifen on MCF-7 breast cancer cells. The bioinformatics analysis found that the CarG element existed in the pro-moter region of COMT gene of many familiar verte-brates, including of human, rhesus macaque, chimpanzee, etc. The results of RT-PCR assay further showed that MRTF-A could enhance the transcrip-tion level of COMT. These results are the first to indicate that COMT might be a target gene which could be regulated by MRTF-A/SRF, and such transactivation event might be involved in the process of tamoxifen resistance.