TITLE:
Activity Induced by a Naphthalene-Prazosin Derivative on Ischemia/Reperfusion Injury in Rats
AUTHORS:
Betty Sarabia-Alcocer, Lauro Figueroa-Valverde, Francisco Díaz-Cedillo, Lenin Hau-Heredia, Marcela Rosas-Nexticapa, Elodia García-Cervera, Eduardo Pool-Gómez, Rolando García-Martínez, Braulio Zepeda-Acosta
KEYWORDS:
Prazosin, Left Ventricular Pressure, Nifedipine, Naphthalene
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.5 No.12,
November
27,
2014
ABSTRACT: In this study, a new naphthalene-prazosin derivative (compound 5) was synthetized with the objective of evaluating its activity on ischemia/reperfusion injury. The Langendorff technique was used to evaluate the effect of the compound 5 on ischemia/reperfusion injury. Additionally, the mechanism of action involved in the activity exerted by the compound 5 on perfusion pressure and coronary resistance was evaluated by measuring left ventricular pressure in absence or presence of following compounds; prazosin, metoprolol, indomethacin and nifedipine. The results showed that the compound 5 reduced infarct size compared with the control conditions. Other results showed that the compound 5 significantly increases (p = 0.05) the perfusion pressure and coronary resistance in isolated rat heart. In addition, other data indicate that the compound 5 increases left ventricular pressure in a dose-dependent manner (0.001 to 100 nM); however, this phenomenon was significantly inhibited by nifedipine at a dose of 1 nM (p = 0.05) and this effect was independent of cAMP levels. In conclusion, these data suggest that the naphthalene-prazosin derivative exerts a cardio protective effect via the calcium channels activation and consequently induces changes in the left ventricular pressure levels. This phenomenon results in a decrease of myocardial necrosis after ischemia and reperfusion.