TITLE:
Hormones Regulate in Vitro Organ Regeneration from Leaf-Derived Explants in Arabidopsis
AUTHORS:
Xiangyu Zhao, Guoting Liang, Xingguo Li, Xiansheng Zhang
KEYWORDS:
Arabidopsis, Hormones, In Vitro Organogenesis, Leaf
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.24,
November
27,
2014
ABSTRACT: Plant in vitro organogenesis is
well-controlled and thus provides an ideal system for plant propagation and
studying mechanisms of plant development. However, the data on systematic in vitro organogenesis from leaf explant
with various concentrations and combinations of hormones are limited. Arabidopsis is a very useful model plant
species for many aspects of plant biological study. Here, we reported a simple,
fast and efficient one-step process for evaluating leaf explant-derived in vitro Arabidopsis organogenesis involving the application of various
concentrations and combinations of exogenous hormones. The central portion of
the fourth rosette leaf was harvested from the 21-days-old seedling and
cultured in vitro on the media
containing 216 combinations of exogenous hormones. Different types of organs,
including roots, shoots, inflorescences, and leaf-like organs were initiated
from leaf explants. Several optimal experimental combinations were selected. A
hormone combination, 1.00 μM NAA + 10.00 μM ZT, was considered as the most efficient
one for adventitious shoot regeneration. And for adventitious root
regeneration, six hormone combinations, [(NAA + ZT: 1.00 + 0.10 μM; 10.00 +
0.01 μM; 20.00 + 0.10 μM; 20.00 + 1.00 μM) and (NAA + 6-BA: 10.00 + 0.10 μM;
20.00 + 10.00 μM)], were thought to be the best ones. Further, both auxin and
cytokinin ratios and concentrations were crucial for efficient in vitro organogenesis. Our study
provides the important information for hormone-regulated organogenesis.