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Schneider, M.C., Najera, P., Aldighieri, S., Galan, D.I., Bertherat, E., Ruiz, A., Dumit, E., Gabastou, J.M. and Espinal, M.A. (2014) Where Does Human Plague Persist in Latin America? PLoS Negeted Tropical Diseases, 8, e2680.
http://dx.doi.org/10.1371/journal.pntd.0002680

has been cited by the following article:

• TITLE: The Development of a Loop-Mediated Isothermal Amplification (LAMP) Procedure for Plague Diagnostic

  AUTHORS: Mariana de Lira Nunes, Carina Lucena Mendes-Marques, Alzira Maria Paiva de Almeida, Nilma Cintra Leal

  KEYWORDS: Plague, Yersinia pestis, Diagnosis Tests, Loop-Mediated Isothermal Amplification (LAMP)

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.5 No.16, November 25, 2014

  ABSTRACT: Plague caused by Yersinia pestis is one of the infectious diseases subject to the International Health Regulations (IHR). Permanent monitoring of the focal plague areas is mandatory in order to enable prompt control measures to prevent the spread of the disease. Therefore, the availability of efficient diagnosis tests is of paramount importance. Here, we describe a loop-mediated isothermal amplification (LAMP)-based procedure for rapid Y. pestis detection. We constructed a set of LAMP primers, which were used in assays to establish the reaction conditions that would lead to the quick visualization of the results by evaluating the test tube with the naked eye. The primers were specifically designed to target the caf1 gene located on pFra/Tox (pMT), a prototypical plasmid of Y. pestis. The LAMP procedure was performed at 65°C for 45 min in a water bath and allowed for the detection of at least 10 pg of bacterial DNA. Due to its simplicity, specificity, sensitivity and rapidity, the LAMP technique is an additional tool that may be implemented in routine plague diagnoses, especially in emergencies.