TITLE:
Larvicidal Properties of Botanical Extracts of Lawsonia inermis against Anopheles stephensi
AUTHORS:
Hasan Bakhshi, Mohammad Reza Abai, Gholamreza Amin, Rasoul Zolfi, Masoumeh Pirmohammadi, Azam Bakhshi, Fakhredin Taghinezhad, Seyed Hasan Moosa-Kazemi
KEYWORDS:
Anopheles stephensi, Lawsonia inermis, Larvicidal Properties
JOURNAL NAME:
Advances in Infectious Diseases,
Vol.4 No.4,
November
24,
2014
ABSTRACT: The aim of this study was to determine the
larvicidal activity of Lawsonia inermis against Anopheles stephensi as the main
malaria vector in Iran. This study was carried out from February to July 2011.
Larvicidal activity of >L. inermis was studied in the range of 4 - 4000 PPM in
the laboratory against early and late stages of larvae of An. stephensi. The
larvae were reared in theinsectarium. The LC50 and LC90 values of the
larval stages of An. stephensi were calculated by probit analysis and
regression line draw using Microsoft office excel 2003 software. The highest
toxic effect of L. inermis was found at 4000 PPM and the lowest at 4 PPM
against larval stages I and II. The same result was found against larval stages
III and IV. The LC50 and LC90 was found as 413.8 and 3366.3 respectively
against larval stages I and II while against late stages found as 696.9 and
3927.7 respectively. This study suggests that L. inermis extract can be used as
an alternative larvicidal compound during the IPM programs for the An.
stephensi control. It is recommended to investigate the competency of other
similar plants to malaria control.