TITLE:
Nucleotide Sequencing and Phylogenetic Analysis Using PCR Amplicons of U3 Gene of Jaagsiekte Sheep Retrovirus (JSRV) Detected in Natural Cases of Ovine Pulmonary Adenocarcinoma in India
AUTHORS:
Vemuri Rama Devi, Egi Janardhan Yadav, Tumati Sreenivasa Rao, Kokila Satheesh, Paila Suresh, Betala Baby Manasa
KEYWORDS:
OPA, JSRV, Sequencing, Phylogenesis, India
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.4 No.11,
November
19,
2014
ABSTRACT: Ovine pulmonary adenocarcinoma (OPA) caused
by an exogenous Jaagsiekte sheep retrovirus (JSRV) is prevalent in Indian
sheep. In the present study, OPA was diagnosed in sheep by clinical signs,
gross and histopathology and polymerase chain reaction (PCR). Proviral DNA of
exogenous JSRV was detected in lung tumor tissues, mediastinal lymph nodes,
blood and lung fluid samples from natural cases of OPA by using U3-hn PCR and
the PCR amplicons were sequenced to analyze nucleotide divergence. In total,
six isolates were sequenced that had 96% - 100% homology with a UK strain
(AF105220.1) but more divergent from a South African strain (M80216) with 88% -
93% identity. The phylogenetic analysis revealed segregation of the six
isolates into two clusters. In conclusion, this study is the first report on
sequencing and phylogenetic analysis of JSRV in India and further studies are
suggested to know the complete sequencing and genetic divergence of JSRV in
Indian sheep.