TITLE:
Colon Adenocarcinoma Diagnosis in Human Samples by Multicontrast Nonlinear Optical Microscopy of Hematoxylin and Eosin Stained Histological Sections
AUTHORS:
Javier Adur, Mariana Bianchi, Vitor B. Pelegati, Silvia Viale, María F. Izaguirre, Hernandes F. Carvalho, Carlos L. Cesar, Víctor H. Casco
KEYWORDS:
Nonlinear Microscopy, Nonlinear Multicontrast, Histological Sections, Human Colon Cancer
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.5 No.13,
November
17,
2014
ABSTRACT: Combined multimodal nonlinear optical
(NLO) microscopies were used to detect and quantify morphological changes
associated with stroma and epithelial transformation in colon cancer. Our
findings provide complementary information about tissue microstructure,
displaying distinctive patterns between normal and malignant human colon.
Additionally, we have demonstrated the usefulness of using fixed tissues for
the disease diagnostic and prognostic.The present work provides a
framework for using NLO techniques as a clinical diagnostic tool for human
colon cancer. NLO metrics could be applied to other disorders, which are
characterized by abnormal cell proliferation and collagen assembly.