TITLE:
Fourier Transform Infrared Spectroscopy Typing an Enterococcus sp.
AUTHORS:
Mohammad I. Abu Taha, Hatem K. Eideh, Sameeh M. Saed, Hazem Jaber
KEYWORDS:
Enterococci, Antibiotic Susceptibility Testing, FTIR Spectroscopy, PCR, VRE Typing
JOURNAL NAME:
Journal of Modern Physics,
Vol.5 No.16,
October
30,
2014
ABSTRACT: Enterococcus species are one of the leading causes of nosocomial
infections, which are difficult to treat specially with the rise of its Vancomycin
resistant. Studies of Enterococcus isolates
are essential for epidemiological investigation. Typing Enterococci is often based on the traditional phenotypic as well as
genotypic methods. In this study Fourier-transform infrared (FTIR) spectroscopy
is used as a novel phenotypic approach to the typing of Enterococci. FTIR
spectroscopy results compared to antibiotic susceptibility testing and PCR amplification
of Vancomycin gene results; the analysis showed that, 6 isolates were positive for
Van gene (4 of VanA, 1 of VanB and 1 VanA plus VanB). Three of VanA and VanA plus
VanB were resistant to all antibiotic tested (Ampicillin, Teicoplamin and Vancomycin) and VanB was found to be
sensitive. FTIR spectroscopy (first derivatives) divided the isolates into 8 groups.
3 groups of VanA (4 isolates), one of VanB (one isolate), one of VanA plus VanB
(one isolate) and the other 13 Enterococcus isolates were divided into 3 clusters. The study demonstrated that FTIR spectroscopy
has good discriminative capacity and high reproducibility as compared to other techniques.