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Pelicano, H., Zhang, W., Jinyun, L., Hammoudi, N., Dai, J., Rui-Hua, X., Lajos, P. and Huang, P. (2014) Mitochondrial Dysfunction in Some Triple-Negative Breast Cancer Cell Line: Role of mTOR Pathway and Therapeutic Potential. Breast Cancer Research, 434, 1-16.

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• TITLE: Sensitivity Evaluation of Two Human Breast Cancer Cell Lines to Tamoxifen through Apoptosis Induction

  AUTHORS: Spencer Keene, Charles Azuelos, Shyamal K. Majumdar

  KEYWORDS: Tamoxifen, Apoptosis, MCF-7 and MDA-MB-231 Human Breast Cancer Cell Lines, Mitochondrial Membrane Potential Assay, Estrogen Receptor

  JOURNAL NAME: Open Journal of Apoptosis, Vol.3 No.4, October 24, 2014

  ABSTRACT: Tamoxifen citrate (TAM) has been used to treat breast cancer in women for many years. The com-parative effects of TAM in inducing apoptosis were evaluated in estrogen receptor-positive (ER- positive MCF-7) and estrogen receptor-negative (ER-negative MDA-MB-231) human breast cancer cell lines in vitro in order to determine if these two cell lines differ in their sensitivity to TAM. Mi-tochondrial membrane permeability potential disruption was assessed in both cell lines by a lip-ophilic cationic dye (DePsipher assay, Trevigen, Inc.) utilizing fluorescence microscopy. Using this specific fluorochrome, we were able to associate mitochondrial membrane disruption to early, mid-, and late apoptotic cells. TAM induced cell death via apoptosis in both ER-positive and ER- negative cells, however, apoptosis induction was more pronounced in ER-positive MCF-7 compared to ER-negative MDA-MB-231 breast cancer cells. These findings may have some therapeutic use in the treatment of estrogen dependent and estrogen independent breast cancer.